With the help of a detective game, Kenneth Wallace-Müller from the Gene Jury team introduces the use of DNA in forensics and the ethical questions involved.
Peter has been found dead in his hotel room. Who could have killed him? What DNA evidence can you find at the crime scene and how can you analyse it? Can you find the murderer?
The game is most suitable for students aged 10-15 [note that the reviewer suggested using the activity with older students]. You will need time to print, cut and laminate the resources, 30 minutes to play the game, and additional time for discussion.
Before introducing the game, explain how DNA fingerprinting works. Do not forget to point out the differences between DNA fingerprinting (profiling) and sequencing the complete genome. You may find Hodge & Wegener (2006) and the resources on the Gene Jury websitew1 helpful.
To run the game, you will need the following materials, all of which can be downloaded from the Gene Juryw1 website.
Ideally, all the materials except the worksheets should be printed in colour and laminated.
One student is the police pathologist; give him or her the pathologist’s report card and the DNA profile card of the victim.
Optionally, one student represents the victim: lying on the floor with a knife (or substitute) nearby. Even if you choose to imagine the victim and weapon, place the five DNA evidence cards (the knife blade, the knife handle, the victim’s fingernails, the victim’s jacket, and the blood on the window) around the ‘crime scene’.
The rest of the class are the investigators, working in groups of about four. Give these students the worksheets.
The flow diagram (Figure 5, below) illustrates the game sequence.
Last night in the local hotel, a terrible crime was discovered. Peter, a well-known businessman, was found dead in his hotel room by two guests, Alex and Olivia, at 11pm. They immediately telephoned the police, who arrived soon afterwards. The pathologist examined the body, and estimated the time of death at 9pm, not long after Peter had finished dinner.
Peter had held a dinner party that evening with some friends to celebrate finishing writing, by hand, a book about his life. The party had taken place in the hotel dining room with his five friends, who had all stayed that night in the hotel. After the police arrived, the five guests and the hotel maid were woken, and assembled downstairs to be questioned.
|Type of sample (e.g. blood or skin)||Where was it found?|
|Skin||On the victim’s jacket|
|Name||Notes from suspect’s statement||Do you suspect him / her?||Ask for sample? (Select only three)|
|Sample from the crime scene||Does this profile match any of your suspects? If so, which?||Do you think this sample is from the murderer?|
|Blood on the windowsill|
|Blood on the knife blade|
|Skin cells on the knife handle|
|Skin cells under the victim’s fingernails|
|Skin cells on the victim’s jacket|
After his arrest, Eric decided to confess to the police what happened that night.
In his former life, Eric had been arrested several times for carrying and taking drugs. He had decided to forget his old life, and he now owned his own restaurant. Only a handful of people knew about his past, including Peter. Peter also had a dark past, and had known Eric very well. He decided to write about Eric and include details of his criminal activities in the book of his life story.
On the evening of the murder, Peter was celebrating the completion of his book by having dinner in the hotel with some friends, including Eric. During the conversation over the meal, Eric realised what Peter had written about him in his book, and how it could damage his reputation.
After dinner, all the guests stayed in the restaurant for coffee. Eric finished and went for a walk in the gardens to plan a way to silence Peter and steal his book. He crept into the kitchen and stole a knife, concealing it in his trouser pocket. Eric hatched a plan to meet Peter in his hotel bedroom for a chat, and – when Peter was least expecting it – to kill him using the knife.
Everything went according to his plan, but while Peter lay dying on the floor covered in blood, Eric heard footsteps walking past the room, and as he grabbed the book, he panicked, accidentally dropping the knife.
After quickly walking back to his room, he hid the book in his suitcase in order to destroy it later. He planned to use the excuse of an early business meeting to leave first thing the next morning.
The DNA profiles used in the DNA detective game are loosely based on band patterns from real profiles. Profiling involves forensic scientists looking for markers (small sequences) within DNA, the sizes of which vary between people. By including multiple markers, all with size variations between individuals, it is possible to create an almost unique profile for each person. In early profiles, these marker sizes were shown as bands of different sizes (rather like barcodes), but these days, profiles are now often represented as graphs.
In the UK, 10 pairs of markers plus a sex chromosome marker are analysed to ensure that two given people can effectively be differentiated. This would give a match probability (the chance of finding two people with the same profile) of less than one in a billion (1 000 000 000). The chance of finding a match is higher between family members, whose DNA is shared, and identical twins, who have identical (or nearly identical) DNA profiles. Profiles generated in England and Wales are currently stored in the UK National DNA Database.
The DNA detective game reflects reality in that profiles, loosely represented here as the original ‘barcodes’, are generated for every individual as well as from crime scene stains (such as blood, skin cells or hair). The eight markers used in the game are more than enough to differentiate the six suspects from one another. As in reality, each profile in the game is compared with those of other suspects of a crime being investigated and other profiles found at the crime scene. It can also be compared to a profile database of suspects and criminals and of samples from previous crimes.
The main problems that forensic scientists face in generating real-life profiles are crime scene stains containing DNA from several people, stains containing very small amounts of DNA, or stains containing degraded DNA. These problems can be overcome to an extent by profiling all of the suspects and those who may have visited the crime scene, by developing newer profiling techniques and by using larger numbers of DNA markers.
DNA profiling technology is used as a complement to other investigative techniques and, in court, DNA-based evidence should be backed up with other non-DNA-based evidence, such as video or witness statements.
The game raises several ethical questions about the forensic uses of a DNA database. Below are some questions, together with possible answers, which can be used in a discussion. Remember that there are no correct answers here: governments, doctors, scientists and the general public may all have different opinions.
This game was developed as a collaborative effort by the Gene Jury team – Heather McQueen, Fiona Stewart, Sarah Keer-Keer and Kenneth Wallace-Müller – at the University of Edinburgh, UK. For more details, see the Gene Jury websitew1.
Thanks to Sandra Couperwhite, forensic scientist with Lothian and Borders Police, for her help with the box ‘How well does the game reflect reality?’
To download the materials for the detective game, visit: www.biology.ed.ac.uk/projects/GeneJury/databaseDNAdetectives.html
For more details of DNA profiling, see: www.biology.ed.ac.uk/projects/GeneJury/databaseTeachersResources.html
For a list of more materials about DNA on the Gene Jury website, see: www.biology.ed.ac.uk/projects/GeneJury/learningzone_whoseDNA.html
Gardner G (2006) The detective mystery: an interdisciplinary foray into basic forensic science. Science in School 3: 35-38.